Citation: Chandrashekar DS, Bashel B, Balasubramanya SAH, Creighton CJ, Rodriguez IP, Chakravarthi BVSK and Varambally S. UALCAN: A portal for facilitating tumor subgroup gene expression and survival analyses. Neoplasia. 2017 Aug;19(8):649-658. doi: 10.1016/j.neo.2017.05.002 [PMID:28732212]
Step 1: Go to analysis page of UALCAN
Step 2: Choose cancer of interest from left panel by clicking it.
Step 3: UALCAN lists top 25 up-/down-regulated genes in cancer of interest compared to normal samples.
Step 4: User can analyze gene expression and survival profiles of individual gene by clicking the on the heat map.
Step 2: Enter offical symbol of gene(s) in the text area.
Step 3: Choose the TCGA data set of interest from the drop down menu.
Step 4: Then click explore to query UALCAN for input genes.
Step 5: UALCAN lists the input genes and facilitates users to analyze expression and survival profiles of the input genes.
Obtaining EZH2 expression profile in breast invasive carcinoma dataset based on patient’s race.
Downloading the box plot from UALCAN
Analyzing survival plots of EZH2 in prostate adenocarcinoma TCGA dataset
Step 2: Query UALCAN for pre-compiled gene classes
Step 3: Select gene class of interest (e.g. Kinase coding genes) and click “Explore” to see results
Step 4: UALCAN lists all kinase coding genes and indicates their expression status and survival effect in different cancers.
Cancer with red border show up regulation of gene
Cancer with green border show down regulation of gene
Step 5: User can click on any of these link to visualize expression and survival profiles